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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in lots of tumors, which leads to chromosome instability, and thus might lead to tumorigenesis. This protein was found to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important position in promoting mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two added splice variants had been noted but detailed nucleotide sequence information is not really still obtainable.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide commonly contains the epitope recognized with the antibody.
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This guideline outlines additional achievable optimization of cellular and immunoassay parameters to be certain the best possible results are...
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best final results are...
Antibodies bound to the blocking peptide no more bind for the epitope on the focus on protein. By evaluating the staining through the blocked antibody versus the antibody by yourself, you can see which staining is particular.
Concentrate on Info Cyclin E belongs to your extremely conserved cyclin relatives, whose members are characterized by a spectacular periodicity in protein abundance in the cell cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation designs which lead to your temporal coordination of each and every mitotic party. Cyclin E types a complex with and functions like a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S changeover. This protein accumulates at the G1-S section boundary and is particularly degraded as cells development as a result of S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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